PIN1 Knockout cell line (HGC-27)

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PIN1 Knockout cell line (HGC-27) Order

PIN1 Knockout cell line (HGC-27)
Catalog#
YKO-H864
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIN1 Knockout cell line (HGC-27)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PIN1 Knockout cell line（HGC-27）
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
PIN1
Gene ID
5300
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_D9W8(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript PIN1-201)
Validation: PCR+Sanger Sequencing
PIN1 Knockout cell line (HGC-27) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HGC-27		 STR Info (Cell bank)
Cell Line:HGC-27
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 111213.0111213.0
D16S539 10111011
VWA 14141414
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2021
FGA 2222
D2S1338 2224
D21S11 303334.0
D18S51 1617
D8S1179 1111
D3S1358 1717
D6S1043 1818
PENTAE 1818
D19S433 1414
PENTAD 913
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HGC-27 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIN1 gene knockout PIN1 Knockout cell line（HGC-27） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PIN1 Knockout cell line (HGC-27)
PIN1 Knockout cell line (HGC-27)
Catalog#
YKO-H864
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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